Quantitative Measurement of Lactoferrin in stool
The IDK Lactoferrin ELISA represents an advanced enzyme immunoassay designed for the accurate quantification of lactoferrin, a well-established biomarker indicative of neutrophilic intestinal inflammation, specifically in stool samples. This assay plays a pivotal role in supporting the monitoring of disease activity in inflammatory bowel disease (IBD), predicting relapses in IBD, and assessing treatment responses within the context of IBD management.
Sample Extraction Procedure
To initiate the analysis, homogenised stools are subjected to extraction using the versatile Stool Sample Application System containing IDK Extract® universal stool extraction buffer, which also finds applicability in various other faecal assays. This extraction process is characterised by its efficiency, cleanliness, and precision, providing a ready-to-analyse sample in minutes. This streamlined process allows for the rapid pre-analytical stages of lactoferrin measurement and automation of the assay itself.
The extracted stool samples demonstrate stability at different temperature ranges, maintaining their integrity at room temperature (15-30 °C), 2-8 °C, and even at -20 °C for a period of 9 days. This extended stability period enhances the convenience of sample handling and allows for flexibility in the laboratory workflow. The versatility of the IDK Lactoferrin ELISA, coupled with the efficiency of the stool sample extraction process, contributes to the reliability and effectiveness of lactoferrin quantification in the context of gastrointestinal health assessments.