Faecal Lactoferrin ELISA (IDK®)

Product code: K6870 (96 wells)

Quantitative Measurement of Lactoferrin in stool

The IDK Lactoferrin ELISA represents an advanced enzyme immunoassay designed for the accurate quantification of lactoferrin, a well-established biomarker indicative of neutrophilic intestinal inflammation, specifically in stool samples. This assay plays a pivotal role in supporting the monitoring of disease activity in inflammatory bowel disease (IBD), predicting relapses in IBD, and assessing treatment responses within the context of IBD management.

 

Sample Extraction Procedure

To initiate the analysis, homogenised stools are subjected to extraction using the versatile Stool Sample Application System containing IDK Extract® universal stool extraction buffer, which also finds applicability in various other faecal assays. This extraction process is characterised by its efficiency, cleanliness, and precision, providing a ready-to-analyse sample in minutes. This streamlined process allows for the rapid pre-analytical stages of lactoferrin measurement and automation of the assay itself.

The extracted stool samples demonstrate stability at different temperature ranges, maintaining their integrity at room temperature (15-30 °C), 2-8 °C, and even at -20 °C for a period of 9 days. This extended stability period enhances the convenience of sample handling and allows for flexibility in the laboratory workflow. The versatility of the IDK Lactoferrin ELISA, coupled with the efficiency of the stool sample extraction process, contributes to the reliability and effectiveness of lactoferrin quantification in the context of gastrointestinal health assessments.

 

Key Advantages

Simple, fast and reliable, with a total incubation time of just 70 minutes
One extract for multiple assays; lactoferrin, calprotectin, zonulin, pancreatic elastase, faecal immunochemical test, faecal bile acids
Easily automated on any open ELISA automate, such as the Dynex DS2

Specifications

ELISA

41 tests

Storage: 2-8 °C

Stool sample

Faecal Lactoferrin ELISA FAQs

What is the IDK Lactoferrin ELISA used for?

The IDK Lactoferrin ELISA is an advanced enzyme immunoassay designed for the quantitative determination of lactoferrin in stool samples. Lactoferrin serves as a biomarker for neutrophilic intestinal inflammation.

Why is Lactoferrin measurement important?

Lactoferrin measurement is crucial for monitoring disease activity in inflammatory bowel disease (IBD), predicting relapses, and assessing treatment responses. It provides valuable insights into the inflammatory status of the intestines.

What is the role of stool sample extraction?

Stool sample extraction involves preparing homogenised stool samples using the IDK Extract® universal stool extraction buffer so that the sample is ready to test.

How is the stool sample extraction process performed?

The extraction process is fast, clean, and precise, utilising the IDK stool sample application system. Using the dipstick, exactly 15mg of stool can be extracted from 3 different parts of a raw stool sample by making sure all the notches in the dipstick are filled with sample. The dipstick with the attached stool is then placed back into the sample tube. Excess material will be stripped off by the blue cone-shaped insert, letting 15mg of sample into the tube. The tube, which contains a universal extraction buffer – IDKExtract®, is then shaken well until no stool sample remains in the notches and the stool is completely suspended in the extraction buffer. This system allows for the rapid preparation of dilutions, optimising samples for subsequent lactoferrin analysis.

Can the IDK Extract® universal stool extraction buffer be used for other assays?

Yes, the IDK Extract® universal stool extraction buffer is versatile and can be applied to numerous faecal assays, (e.g. calprotectin, pancreatic elastase, faecal bile acids, FIT, zonulin, alpha-1-antitrypsin) adding to its utility in gastrointestinal research and diagnostics.

What is the stability of lactoferrin after the extraction process?

Stool extracts remain stable at different temperature ranges, including room temperature (15-30 °C), 2-8 °C, and -20 °C for a period of 9 days. This stability facilitates convenient sample handling and flexibility in laboratory workflows.

Is the IDK Lactoferrin ELISA suitable for testing stool samples that have been held in long-term storage?

Lactoferrin is stable in raw stool if stored at -20°C for up to 6 months. Samples can be thawed and extracted for testing within this period.

How does Lactoferrin quantification contribute to the management of IBD?

Lactoferrin quantification aids in monitoring disease activity, predicting relapses, and evaluating treatment responses in inflammatory bowel disease, providing valuable information for effective patient management.

Is the IDK Lactoferrin ELISA user-friendly for laboratories?

Yes, the IDK Lactoferrin ELISA is designed for ease of use in laboratories. It can be easily automated on any open ELISA automate and is simple, fast and reliable, with a total incubation time of just 70 minutes.


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