- Disease area
- Laboratory services
The IDK® faecal bile acids assay is an innovative method that enables the quantitative measurement of bile acids in stool samples. This photometric assay is rapid and can be helpful in diagnosing bile acid diarrhoea in patients with chronic or persistent loose or liquid stools. It can be utilised to diagnose various patient groups, such as those with irritable bowel syndrome (IBS), Crohn’s disease, post-cholecystectomy, and coeliac disease.
The IDK® faecal bile acids assay is a quick, inexpensive, and non-invasive method for the routine differential diagnosis of chronic diarrhoea. This 96-well colorimetric assay allows for the quantitative measurement of total free bile acids in stool samples in just 9 minutes. The patient does not undergo any radiation exposure and does not require any special preparation or follow-up visits. A stool sample can be easily obtained at home and sent to the laboratory for analysis. Stool extracts remain stable for 3 days at room temperature (15-30°C), and for 7 days at 2-8°C and 14 days at -20°C.
The laboratory process is made more efficient by employing our rapid extraction tubes containing a universal extraction buffer, allowing for the analysis of the stool sample in multiple assays if necessary. The IDK Extract® universal stool extraction buffer is used to extract homogenized stools for various faecal assays. The Rapid Extraction Tubes (IDK Stool Sample Application System) are used to create dilutions for analysis, making the extraction process fast, accurate, and hygienic.
The system comprises a pre-filled tube containing 1.5ml of IDK Extract® extraction buffer and a sampling stick. After sampling the fresh stool, by pushing the stick through the tube collar, exactly 15mg of stool sample is obtained, ensuring a constant dilution factor of 1:100. This system facilitates the rapid, precise, and hygienic extraction of stool samples, with reproducible results. Additionally, the tubes can be directly loaded onto lab automates for further dilution and analysis.
The rapid stool extraction tubes enable the examination of a single stool sample for multiple analytes and may serve as the foundation for efficient patient care coordination between primary and secondary healthcare providers. Patients who exhibit diarrhoea symptoms for example would have their stool samples tested for both FBAs and faecal calprotectin prior to referral for colonoscopy, if deemed necessary.
The IDK® faecal bile acids assay offers several benefits for clinicians, healthcare professionals and their patients. It is a quick and non-invasive method that helps in the investigation and diagnosis of bile acid diarrhoea and supports the differential diagnosis of bile acid malabsorption. The test is convenient for patients and simple for providers, delivering quantitative results in just 9 minutes. This is aided by the use of rapid sample collection and extraction tubes with a universal extraction buffer. This enables fast, accurate, clean, and reproducible extraction of stool samples, ready to analyse using the faecal bile acids test as well as several other faecal assays on the same extract (such as faecal calprotectin and pancreatic elastase) if indicated. The stool extracts remain stable for up to 3 days at room temperature, allowing for convenient storage and transport. Overall, the IDK® faecal bile acids assay is a simple and effective method for the routine differential diagnosis of chronic diarrhoea.
In summary, measuring faecal bile acids can provide useful information about digestive health and disease risk in patients with chronic diarrhoea. If patients have concerns about their digestive function or are experiencing symptoms such as diarrhoea with no explanation, consider faecal bile acid testing.
Storage: 2-8 °C
Stool samples can be extracted directly and efficiently using the IDK Stool Sample Application System, which allows easy, clean and precise preparation of stool sample extracts.
Stool samples can be extracted directly and efficiently using the IDK Stool Sample Application System, which allows easy, clean and precise preparation of sample extracts.
The IDK Pancreatic Elastase ELISA is a quantitative enzyme immunoassay for the determination of pancreatic elastase in stool samples. This supports the diagnosis of exocrine pancreatic insufficiency in a range of cases including unexplained diarrhoea, gut microbiota, dysbiosis, IBS/IBD, steatorrhoea, flatulence, weight loss, upper abdominal pain and dietary intolerances.