Faecal Haemoglobin ELISA (IDK®)

Product code: K7816D (96 wells)

Quantitative Enzyme Immunoassay for Haemoglobin

The IDK Faecal Haemoglobin ELISA stands as a cutting-edge enzyme immunoassay designed to deliver precise quantitative measurements of human haemoglobin in stool samples. This assay serves as a crucial marker for identifying early colorectal cancer and adenomatous polyps by detecting gastrointestinal bleeding, particularly originating from colorectal adenomas and tumours. The intermittent presence of blood in stools, whether in symptomatic or asymptomatic individuals, acts as a key indicator for further investigation, often necessitating colonoscopy to pinpoint the lesion or source of bleeding.

 

Stool Sample Extraction Process

Efficient stool sample extraction is facilitated through the utilisation of the Stool sample application system – a rapid, precise and hygienic sample extraction tube prefilled with IDKExtract® universal stool extraction buffer, which holds the added advantage of compatibility with numerous other faecal assays. The extraction process is streamlined and hassle-free, employing rapid extraction tubes to homogenise stools, create dilutions, and prepare samples for analysis. This innovative system ensures a fast, clean, and precise extraction process, contributing to the reliability of results.

Moreover, the extracted stool samples demonstrate remarkable stability under various storage conditions. Whether stored at room temperature (15-30 °C), in refrigerated conditions (2-8 °C), or even frozen at -20 °C, the stool extracts maintain their integrity for a period of seven days. This robust stability allows for flexibility in the analysis timeline, helping to overcome the challenge of analyte degradation in native stool, and offering convenience to laboratories and healthcare professionals alike.

In essence, the IDK Faecal Haemoglobin ELISA, coupled with its efficient stool sample extraction system, not only provides accurate quantitative data on haemoglobin levels but also ensures a seamless and reliable process, supporting the early detection and diagnosis of gastrointestinal bleeding with the potential to guide subsequent medical interventions.

Key Advantages

Simple, fast and reliable, with a total incubation time of just 130 minutes
Highly specific for human haemoglobin
No cross-reactivity with pancreatic amylase, myeloperoxidase, lysozyme, chymotrypsin and secretory immunoglobulin A
Broad linear range up to 50 µg/g
Easily automated on any open ELISA automate, such as the Dynex DS2

Specifications

ELISA

41 tests

Storage: 2-8 °C

Stool sample

Faecal Haemoglobin ELISA (IDK®) FAQs

What is the IDK Faecal Haemoglobin ELISA, and how does it work?

The IDK Faecal Haemoglobin ELISA is a quantitative enzyme immunoassay designed for the precise determination of human haemoglobin levels in stool samples. It operates by employing enzymatic reactions to quantify haemoglobin, serving as a reliable marker for gastrointestinal bleeding.

Why is haemoglobin measurement in stools important?

Haemoglobin measurement in stool is crucial as it serves as an indicator of gastrointestinal bleeding. Colorectal adenomas and tumours can intermittently cause blood in stools, making this measurement essential for early detection and subsequent medical interventions.

When is colonoscopy recommended based on haemoglobin levels in stools?

Colonoscopy is recommended when elevated haemoglobin levels are detected in stools, indicating the presence of gastrointestinal bleeding. This diagnostic procedure helps locate the source of bleeding, allowing for timely and targeted medical interventions.

How is the stool sample extraction process conducted using IDK Extract® and rapid extraction tubes?

The stool sample extraction process is streamlined with the use of the IDK stool sample application system prefilled with IDKExtract®, a universal stool extraction buffer. The rapid extraction tubes are employed to homogenize stools, create dilutions, and prepare samples for analysis in a fast, clean, and precise manner. Using the dipstick, exactly 15mg of stool can be extracted from the stool by making sure all the notches in the dipstick are covered. The dipstick and sample is then placed into the sample tube which strips off any excess material leaving 15mg of sample to become suspended in the IDKExtract® buffer inside the sealed tube. Then, the sample tube is shaken well until no stool sample remains in the notches of the dipstick and the stool is completely suspended in the buffer ready for use.

Can IDK Extract® be used for other faecal assays?

Yes, IDK Extract® is a versatile universal stool extraction buffer that can be utilized for numerous other IDK faecal assays, such as calprotectin, pancreatic elastase, alpha-1-antitrypsin, faecal bile acids and many more, enhancing its utility and compatibility in laboratory settings.

What is the stability of stool extracts under different storage conditions?

Stool extracts remain stable for seven days under varied storage conditions, including room temperature (15-30 °C), refrigeration (2-8 °C), and freezing (-20 °C). This stability provides flexibility in the analysis timeline, accommodating the needs of laboratories and healthcare professionals.

How does the IDK Faecal Haemoglobin ELISA contribute to early detection and diagnosis?

By accurately quantifying haemoglobin levels in stool samples, the IDK Faecal Haemoglobin ELISA plays a pivotal role in the early detection and diagnosis of gastrointestinal bleeding and colorectal cancer. This information guides healthcare professionals in formulating effective medical interventions.

What benefits does the IDK Faecal Haemoglobin ELISA offer for healthcare practitioners?

The IDK Faecal Haemoglobin ELISA offers healthcare practitioners a reliable and efficient tool for quantifying faecal haemoglobin levels, facilitating early detection, and enabling timely and targeted medical interventions. The streamlined sample extraction process and stability of stool extracts further enhance its practicality in clinical settings.


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