The BIOHIT Total 25OH Vitamin D ELISA is a quantitative immunoenzymatic assay for the determination of 25-hydroxyvitamins D2 and D3 in serum and plasma, to aid the diagnosis of vitamin D deficiency, insufficiency or intoxication.
Detects 25-hydroxyvitamin D2 and D3
25-hydroxyvitamin D2 is dissociated from plasma proteins
Assay time 165 minutes
Automatable
Reportable range 4.4-123 ng/ml
R>0.97, validated against ID-LC/MS-MS Ghent Reference Measurement Procedure
The BIOHIT Total 25OH Vitamin D ELISA is a quantitative assay for 25-hydroxyvitamin D2 and D3 in serum and plasma samples to support the investigaton of Vitamin D deficiency, insufficency and intoxication. The assay is a microplate-based competitive enzyme-linked immunosorbent assay and the kit contains all the necessary reagents for analysis. The assay can be performed manually or by automated ELISA processors.
25OH vitamin D, also known as 25-hydroxyvitamin D, is a measure of the total amount of vitamin D in your body. It’s considered the best indicator of vitamin D status.
A 25OH vitamin D test helps assess a patient’s vitamin D status, which is crucial for bone health, immune function, and overall well-being. It aids in diagnosing deficiencies and guiding appropriate treatment.
It is typically measured through a blood test called the 25OH vitamin D test, which detects the concentration of 25OH vitamin D in the blood.
Optimal levels of 25OH vitamin D can vary depending on individual factors and guidelines from different health organisations. Generally, levels between 30-50 ng/mL (nanograms per millilitre) are considered adequate for most people.
Symptoms of deficiency may include fatigue, muscle weakness, bone pain, and an increased risk of bone fractures. Severe deficiency can lead to conditions like rickets in children and osteomalacia in adults.
