Total 25OH Vitamin D ELISA (BIOHIT)

Product code: 602310.02 (96 wells)

Quantitative assay for 25-hydroxyvitamin D

The BIOHIT Total 25OH vitamin D ELISA is a quantitative immunoassay for the determination of 25-hydroxyvitamins D2 and D3 (25OHD2 and 25OHD3) in serum and plasma, to aid in the diagnosis of vitamin D deficiency, insufficiency or intoxication. Making the correct diagnosis depends on the measurement of both 25OHD2 and 25OHD3, both of which are reliably quantified by this assay.

The 25OH vitamin D test is a microplate-based competitive enzyme-linked immunosorbent assay (ELISA). Samples, controls, and calibrators are added to microtiter plate wells along with incubation buffer, allowing 25OH vitamin D to bind to specific antibodies. After washing to remove unbound components, a mixture of biotin-labelled and streptavidin-labelled compounds is introduced, competing for antibody binding sites. Subsequent steps involve washing, addition of a chromogenic substrate (TMB), and stopping the reaction. The colour intensity, inversely proportional to 25OH vitamin D concentration, is measured to determine levels through calibration curve interpolation.

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Key Advantages

Detects 25-hydroxyvitamin D2 and D3
25-hydroxyvitamin D2 is dissociated from plasma proteins
Assay time 165 minutes
Automatable
Reportable range 4.4-123 ng/ml
R>0.97, validated against ID-LC/MS-MS Ghent Reference Measurement Procedure

Specifications

ELISA

40 tests

Storage: 2-8 °C

Blood sample

More Details

25OH Vitamin D

Vitamin D, vital for human health, comes in two forms: D2 (ergocalciferol) and D3 (cholecalciferol), naturally present in certain foods and commonly added to others. Supplementation is recommended in regions with limited sun exposure. The body primarily circulates D3 as 25OHD3 (calcidiol), crucial for bone health. Both D2 and D3 contribute to overall vitamin D status, which is best assessed by measuring 25OH vitamin D levels in blood. Vitamin D facilitates calcium and phosphorus absorption, impacting bone strength, while deficiency is linked to bone diseases and abnormal calcium levels. Excessive vitamin D intake can lead to kidney and tissue damage. Additionally, vitamin D plays roles in cell growth, immune function, and cognitive health, with emerging research suggesting implications in cancer, diabetes, and pregnancy outcomes.

25OH vitamin D FAQs

What is 25OH vitamin D?

25OH vitamin D, also known as 25-hydroxyvitamin D, is a measure of the total amount of vitamin D in your body. It’s considered the best indicator of vitamin D status.

Why should I test my patients for 25OH vitamin D levels?

A 25OH vitamin D test helps assess a patient’s vitamin D status, which is crucial for bone health, immune function, and overall well-being. It aids in diagnosing deficiencies and guiding appropriate treatment.

How is 25OH vitamin D measured?

It is typically measured through a blood test called the 25OH vitamin D test, which detects the concentration of 25OH vitamin D in the blood.

What are the optimal levels of 25OH vitamin D?

Optimal levels of 25OH vitamin D can vary depending on individual factors and guidelines from different health organisations. Generally, levels between 30-50 ng/mL (nanograms per millilitre) are considered adequate for most people.

What are the symptoms of 25OH vitamin D deficiency?

Symptoms of deficiency may include fatigue, muscle weakness, bone pain, and an increased risk of bone fractures. Severe deficiency can lead to conditions like rickets in children and osteomalacia in adults.

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