IDK® TurbiFIT®

Product Code: TU1031.1 (100 tests)
Product Code: TU1031.2 (200 tests)
Product Code: TU1031.8 (800 tests)

Turbidimetric Faecal Immunochemical Test (FIT) Assay

The IDK TurbiFIT employs a particle-enhanced turbidimetric immunoassay (PETIA) methodology, utilising polyclonal antibodies to accurately quantify human haemoglobin levels in stool samples. Also known as a FIT test this assay serves a dual purpose: it acts as a preliminary screening tool for the detection of faecal occult blood, aiding in the early identification of colorectal cancer in asymptomatic individuals, and it also functions as a diagnostic test for potential bowel diseases in patients presenting with lower abdominal symptoms.

Extraction of Stool Samples

The process of obtaining stool samples is streamlined through the utilisation of the IDK® TurbiTUBE®, designed for convenient, clean, and precise sample preparation. Within this tube, there is 1.5 ml of IDK stool extraction buffer, which ensures a consistent dilution ratio of 1:100 when the stool sample is applied. This prepared sample extract is readily available for analysis and can be directly loaded onto numerous clinical chemistry analysers, facilitating an efficient workflow.

This innovative system not only simplifies the sample preparation process but also allows for the determination of three crucial parameters – calprotectin, pancreatic elastase, and haemoglobin (FIT) – all from a single stool sample extract. This not only saves valuable time but also proves to be a cost-effective approach when investigating patients with non-specific abdominal symptoms. Furthermore, the stability of stool extracts is maintained for up to 7 days when stored at room temperature or between 2-8 °C, offering flexibility in the analysis timeline. Overall, the IDK TurbiFIT assay, coupled with the TurbiTUBE® system, presents a comprehensive and efficient solution for stool sample analysis, contributing to the early detection and diagnosis of various gastrointestinal conditions.

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Key Advantages

Enables rapid and effective screening test for bowel disease and colorectal cancer

Simple, fast and reliable, with short incubation times

Results available in less than 15 minutes, depending on the clinical chemistry analyser

Suitable for high throughput workflows, including continuous loading and random access

Reduced hands-on time using the IDK TurbiTUBE with one extract for three turbidimetric assays – calprotectin, pancreatic elastase and haemoglobin

Broad measuring range (1-100 μg/g)

Good clinical correlation with corresponding ELISAs

Specifications

Turbidimetric assay

100, 200 and 800 test packs

Storage: 2-8 °C

Stool sample

More Details

Turbidimetric FIT Assay Kit Details

IDK® TurbiFIT turbidimetric assays show a good clinical correlation with its corresponding ELISA. The kit comes with 6 ready-to-use calibrators and 2 ready-to-use controls and the extracted stool samples are stable for up to 7 days at room temperature and 2–8°C. The TurbiFIT kit is available in three different pack sizes to fit the needs of every laboratory. We recommend a calibration – using the full set of six calibrators – at least once a month or when the reagent lot is changed. The controls included in the kit should be run each time to provide internal QC and validate the stored calibration curve.

Supported Analysers

Immundiagnostik AG provides application notes for a broad range of clinical chemistry analysers containing information about the assay set-up on each specific platform. In addition, they provide the analyser specific performance characteristics of the assay as well as the application parameters. If no application note is currently available for your specific analyser, we have a team of highly trained experts – our technical support team – which will work with you to bring the application to that analyser. If application notes are available for your analyser, but you still require assistance with the start-up of the assay, then of course that will also be provided.

IDK® TurbiFIT® FAQs

What is a turbidimetric FIT assay?

A Turbidimetric FIT (Faecal Immunochemical Test) Assay is a particle-enhanced turbidimetric immunoassay (PETIA) that employs polyclonal antibodies to measure human haemoglobin levels in stool samples. It is primarily used for detecting traces of blood in stool as a preliminary screening for colorectal cancer and diagnosing organic bowel diseases.

How does the IDK TurbiFIT assay work?

The IDK TurbiFIT utilises polyclonal antibodies in a turbidimetric immunoassay to detect human haemoglobin in stool samples. This provides a reliable method for identifying faecal occult blood, aiding in the early detection of colorectal cancer and diagnosing bowel diseases in asymptomatic and symptomatic patients.

What is the significance of detecting faecal occult blood?

Detection of faecal occult blood is crucial as it serves as an early indicator of colorectal cancer and other organic bowel diseases. The TurbiFIT assay’s ability to identify these traces in stool samples enables timely intervention and increases the chances of successful treatment.

How is stool sample extraction facilitated using the IDK TurbiTUBE®?

The IDK TurbiTUBE® is designed for easy, clean, and precise stool sample preparation. It contains 1.5 ml of IDK stool extraction buffer, ensuring a constant sample dilution of 1:100. This prepared sample can be loaded directly onto the analyser, simplifying the workflow for both practitioners and laboratory technicians.

What parameters can be determined using the IDK TurbiTUBE®?

The IDK TurbiTUBE® allows for the determination of three important parameters – calprotectin, pancreatic elastase, and haemoglobin – all from a single stool sample extract. This not only saves time but also proves to be cost-effective, providing a comprehensive analysis of gastrointestinal health.

How long are stool extracts stable after extraction?

Stool extracts prepared using the IDK TurbiTUBE® system remain stable for up to 7 days when stored at room temperature and between 2-8 °C. This offers flexibility in the analysis timeline and ensures the reliability of results even in the event of delayed sample transport.

Is the turbidimetric FIT assay suitable for both screening and diagnosis?

Yes, the TurbiFIT assay is versatile, serving as a preliminary screening tool for colorectal cancer in asymptomatic patients and as a diagnostic test for possible bowel diseases in individuals exhibiting lower abdominal symptoms.

Can the IDK TurbiFIT assay contribute to cost savings?

Yes, the IDK TurbiFIT assay, combined with the TurbiTUBE® system, allows for the determination of multiple parameters from a single stool sample extract, leading to both time and cost savings in the analytical process.

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