Helicobacter pylori ULTRA-FAST UFT300 Quick Test (BIOHIT)

Product code: 602005PLA (5 tests)
Product code: 602019PLA (50 tests)
Product code: 602017 (positive control)

An ultra-fast urease test for gastric biopsies

As part of upper endoscopy or gastroscopy procedures, urease tests are routinely used to identify the presence of H. pylori in gastric biopsies. The UFT300 Helicobacter pylori quick test is a rapid urease test that has transformed the diagnostic process and reporting of H. pylori infection in endoscopy settings. Unlike other urease tests, the UFT300 H. pylori quick test takes just 5 minutes, delivering reliable results that can be entered directly into your endoscopy reporting system and discussed with patients at the end of their procedure.

Urease enzyme is produced in large quantities by H. pylori, which degrades urea used in the UFT300 H. pylori Quick Test to produce ammonia. When this happens, the pH of the reagent changes and the reagent changes colour. During the gastroscopy, biopsy samples from the antrum and corpus mucosa are promptly examined using this ready-to-use test, allowing for precise results to be obtained in as few as 5 minutes.

Key Advantages

Ultra-FAST and Ready to use, with results in just five minutes
Record results during the endoscopy procedure
Sensitivity 94.3%, Specificity 100%, Accuracy 97.5% (compared to histology and C13 UBT n=1000)
Room temperature storage


Colorimetric test

5 or 50 tests

Storage: 18-25 °C

Biopsy sample

More Details

UFT300 Helicobacter pylori Quick Test process

With the UFT300 Helicobacter pylori quick test, the testing is completed with actionable results within 5 minutes, enabling users to directly input results into their reporting software during the endoscopy appointment. This streamlined process eliminates unnecessary administrative tasks, eliminates paperwork, and allows staff to reclaim valuable time.

To perform the test, a biopsy sample is placed in the designated test plate. A single drop of the UFT300 reagent is added, the plate is sealed and gently swirled horizontally, and the results are read after 5 minutes. A positive result is indicated by a magenta colour, while a negative result is represented by yellow. The test exhibits an impressive accuracy rate of 97.5%, with a sensitivity of 94.5% and a specificity of 100% when compared to histology and the C13 urea breath test (UBT) as gold standards. Furthermore, the UFT300 offers the convenience of room temperature storage within the endoscopy unit, further simplifying its usage.


Helicobacter pylori Quick Tests enhance diagnosis and care

BIOHIT produces two urease tests, both backed by scientific publications demonstrating their accuracy and clinical performance compared to established methods considered as the gold standard. These tests are characterised by their speed, reliability, and convenience, leading to enhanced quality of care and service for patients undergoing endoscopy. By enabling rapid urease testing, patients can undergo testing, obtain results, and receive H. pylori treatment all in a single appointment at the point of care.

Ensuring reliable performance of urease tests

To ensure the consistent and accurate performance of Helicobacter pylori urease tests, BIOHIT also supplies a positive urease control called H. pylori Control+, which is available for daily verification. Prior to testing patient samples, this control solution serves as a reliable method to confirm the test’s functionality. H. pylori Control+ is conveniently packaged in a dropper bottle containing 1 ml of urease solution. With just 1 ml of H. pylori Control+, up to 100 quality control tests can be conducted, guaranteeing the ongoing reliability of your Helicobacter pylori urease tests. The BIOHIT H. pylori Control+ is compatible with all rapid urease tests to enhance quality assurance.

Disease Background

Approximately 30% of individuals in the UK are believed to harbour H. pylori in their stomachs, often without experiencing any symptoms. However, approximately 15% of those individuals may encounter problems associated with the bacterium. H. pylori resides within the mucous layer covering the gastric epithelium in infected individuals. If left untreated, the ongoing inflammatory process caused by chronic H. pylori infections can give rise to atrophic gastritis, a condition strongly associated with peptic ulcer disease and increased risk of gastric cancer – two of the most significant disorders affecting the upper gastrointestinal tract.


Helicobacter pylori ULTRA-FAST UFT300 Quick Test FAQs

What is Helicobacter pylori?

Helicobacter pylori is a spiral shaped gram negative bacteria that can infect the stomach and cause various gastrointestinal conditions. It has been shown to increase the risk of gastric cancer and cause peptic ulcer disease.

What are the symptoms of Helicobacter pylori?

Many people with H. pylori infection do not experience any symptoms. However, some individuals may develop symptoms such as stomach pain, bloating, nausea, vomiting, loss of appetite, weight loss, and in more severe cases, peptic ulcers, or gastritis.

How do you test for Helicobacter pylori?

There are several methods to test for H. pylori infection, and different tests are selected depending on the clinical situation and patient history. Non-invasive blood (antibody) tests are used in screening settings or first line while urea breath tests and stool antigen tests can be used to confirm active infection or successful eradication post treatment. Gastric biopsy tests are used in conjunction with gastroscopy and include histology and rapid urease testing (commonly known as CLO tests). Biopsy samples can also be used to determine the best antimicrobial therapy to treat the infection by culture or molecular tests. The choice of test depends on several factors, including the clinical setting, the patient’s symptoms, age, and medical history.

What are rapid urease tests used for?

Rapid urease tests are types of Helicobacter pylori tests that are used to diagnose the presence of H. pylori infection during upper gastrointestinal endoscopy (gastroscopy). They are also sometimes used in the follow-up of treatment to determine the effectiveness of eradication therapy.

What other types of tests are available for detecting H. pylori?

There are several types of tests available, for several sample types, including urea breath tests, stool antigen tests, antibody tests, and gastric biopsy tests via histology, rapid urease testing, culture or PCR.

How does a rapid urease test work?

Rapid urease tests diagnose the presence of H. pylori in a biopsy specimen by detecting urease enzyme activity produced by the bacteria. The test involves placing a biopsy sample in a test plate, adding a reaction buffer, and observing a colour change to determine the result.

How long does it take to get the test results?

The BIOHIT UFT300 H. pylori Quick Test is an ultra-fast urease test that delivers results within 5 minutes, allowing for fast diagnosis during gastroscopy procedures.

What does it mean if patients test positive for H.pylori?

A positive urease test result for H. pylori indicates the presence of the bacteria in the stomach. It suggests that the individual has an active H. pylori infection that may be the cause of their symptoms, and the patient should be considered for treatment to eradicate the bacteria and prevent potential complications.

How accurate is the BIOHIT UFT300 H. pylori Quick Test?

Studies have shown that the BIOHIT UFT300 H. pylori Quick Test has high accuracy, with sensitivity and specificity rates that are comparable to gold standard methods like histology or urea breath tests.

Does antacid or PPI therapy affect rapid urease tests?

Antisecretory therapies including PPIs and H2RA have been shown to reduce the sensitivity of urease tests. This is thought to be caused by altered distribution of bacteria in the stomach as a result of prolonged use of such therapies. Patients should pause antisecretory therapies prior to testing.

Is there a quality control available for rapid urease tests?

Yes, H. pylori Control+ is a positive urease control designed to verify the performance of rapid urease tests daily. These controls help ensure the reliability of the test results and help demonstrate high quality assurance for endoscopy services.

Are Helicobacter pylori tests suitable for all patients?

Helicobacter pylori tests can be used on patients of various ages. However, specific test methods and sample collection techniques may vary depending on the patient’s condition and age.

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    Helicobacter pylori Quick Test (BIOHIT)

    The BIOHIT H. pylori Quick Test is a rapid, one-step urease test to detect H. pylori infection from a biopsy sample during gastroscopy. It can also be used to evaluate the success of eradication therapy. This easy-to-use, gel-based test employs a simple colour indicator that allows easy visual confirmation of positive results in as little as two minutes – and negative results after 30 minutes – without specialist training.