Zonulin (Serum) ELISA (IDK®)

Product code: K5601 (96 wells)

Measurement of Zonulin Levels in Serum

The IDK Zonulin (Serum) ELISA is a state-of-the-art quantitative assay designed for the precise determination of zonulin levels in serum. Zonulin, a pivotal protein in intestinal barrier function, emerges as a promising marker associated with the phenomenon commonly referred to as “leaky gut” syndrome. Our advanced competitive ELISA employs a biotinylated zonulin tracer and polyclonal anti-zonulin antibodies, ensuring a straightforward yet highly effective method for quantifying zonulin concentrations in serum samples.

Zonulin plays a crucial role in regulating the integrity of the intestinal barrier, influencing the permeability of the gut lining. Elevated levels of zonulin have been implicated in conditions where the gut barrier becomes compromised, allowing substances to pass through more easily. Monitoring zonulin levels is integral to gaining insights into the integrity of the gut barrier and assessing potential associations with various health conditions.

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Key Advantages

Quick and reliable, with a total incubation time of just 130 minutes

Highly specific with no cross reactivity with human haptoglobin

Easily automated on any open ELISA automate, such as the Dynex DS2

Specifications

ELISA

41 tests

Storage: 2-8 °C

Blood sample

More Details

IDK Zonulin Assay Principle

The IDK Zonulin (Serum) ELISA is based on the zonulin sequence as published by Wang (Journal of Cell Science, 2000) and di Pierro (Journal of Biological Chemistry, 2001) and operates on a competitive principle. It involves the interaction between a biotinylated zonulin tracer and polyclonal anti-zonulin antibodies, resulting in a precise measurement of zonulin concentration in serum. This method offers accuracy and reliability in the quantitative analysis of zonulin, providing valuable data for researchers and healthcare professionals.

The measurement of zonulin family peptides (ZFP) correlates well with established metabolic traits linked to increased gut permeability, such as insulin resistance and obesity. Zonulin has been identified as a key player in the context of “leaky gut” syndrome, where increased intestinal permeability may contribute to various health issues. Our ELISA assay empowers researchers to explore the potential links between zonulin levels and conditions associated with altered gut barrier function.

Built on simplicity and efficiency, the IDK Zonulin (Serum) ELISA facilitates a seamless workflow for researchers and clinicians. The combination of biotinylated zonulin tracer and polyclonal anti-zonulin antibodies ensures a precise, robust and user-friendly approach to quantitative zonulin analysis.

Zonulin Serum ELISA FAQs

What is Zonulin?

Zonulin is a human protein analogue to the zonula occludens toxin derived from Vibrio cholerae which regulates tight junctions of the digestive tract. Zonulin binds to a specific receptor on the surface of intestinal epithelia and triggers a cascade of biochemical events which induces tight junction disassembly and a subsequent permeability increase of the intestinal epithelia, allowing some substances to pass through and activate immune reactions.

What is the IDK Zonulin (Serum) ELISA used for?

The IDK Zonulin (Serum) ELISA is a quantitative assay designed for the precise determination of zonulin family peptides (ZFP) levels in serum, offering insights into the integrity of the intestinal barrier.

Why is zonulin measurement important in gastrointestinal health?

Zonulin is a key protein regulating the integrity of the intestinal barrier. Monitoring zonulin levels is crucial for understanding gut health and its potential implications in conditions such as “leaky gut” syndrome.

What role does zonulin play in "leaky gut" syndrome?

Zonulin is implicated in conditions associated with increased intestinal permeability, often referred to as “leaky gut” syndrome. This assay allows researchers to explore the potential links between zonulin family peptides (ZFP) levels and altered gut barrier function.

How can researchers benefit from using the IDK Zonulin (Serum) ELISA?

Researchers can leverage this ELISA to quantitatively analyse zonulin family peptides (ZFP) levels in serum, gaining valuable data for studies related to gut health, barrier function, and their associations with various medical conditions.

Is the assay user-friendly for laboratory professionals?

Yes, the IDK Zonulin (Serum) ELISA is designed for efficiency and simplicity, ensuring a user-friendly experience for laboratory professionals conducting quantitative zonulin analysis. It can be easily automated on any open ELISA automate and is quick and reliable, with a total incubation time of just 130 minutes.

Can this assay be applied to clinical research and patient diagnostics?

Yes, the IDK Zonulin (Serum) ELISA is applicable to both research and clinical settings, providing a quantitative tool for investigating zonulin levels in serum samples.

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The IDK Zonulin (Stool) ELISA is a quantitative assay designed for the precise determination of zonulin levels in stool samples. Zonulin, a protein intricately involved in intestinal barrier function, has garnered attention as a potential marker for the phenomenon commonly known as “leaky gut” syndrome.