This MAb identifies the Mr-250,000 and Mr-180,000 human tenascin polypeptides in immunoprecipitation, or just the Mr-250,000 polypeptide in immunoblots. It can also interact with the fibrinogen-like knob domain of the tenascin protein, especially in paraffin sections of human tissue. It can be used in immunofluorescence, immunoblotting and immunohistochemistry on formalin-fixed or paraffin-embedded sections. Tenascin polypeptides isolated from the spent culture supernatant of human fibroblasts by affinity chromatography are used as immunogens for antibody production.
