BIOHIT Archives

Vitronectin

This MAb is specific to human tissue extracellular matrix components such as vitronectin, with no cross-reaction with other matrix molecules. It can be used in immunohistochemical staining, immunoblotting and other immunoassays for cellular and tumour biology research. Purified vitronectin from human plasma is used as an immunogen for antibody production.

Vinculin

This human cytoskeletal polypeptide MAb can be used in immunoblotting, immunohistochemical staining and other immunoassays for cellular and tumour biology research. In immunoblotting, it identifies a 130 kD polypeptide. Vinculin, purified from human smooth muscle tissue, is used as an immunogen for antibody production.

Vimentin

This human cytoskeletal polypeptide MAb recognises vimentin with an Mr of 57,000 in mesenchymal cells, and can be used in immunohistochemical staining and western blotting. Purified vimentin, obtained via electrophoresis from cultured human fibroblasts, is used as an immunogen for antibody production.

Tenascin-C – IgG1, clone EB2

This human extracellular MAb interacts with the fourth and fifth fibronectin-like repeats of human tenascin-C polypeptides with an Mr of 250,000 and 180,000. It can cross-react with humans, guinea pigs and rabbits. It can also be used in immunohistochemical staining, western blotting and other immunoassays for cellular and tumour biology research, as well as for quantitation of Tenascin-C in body fluids. Purified tenascin-C from human fetal fibroblasts is used as an immunogen for antibody production.

Tenascin-C – IgG2a, clone DB7

This MAb identifies the Mr-250,000 and Mr-180,000 human tenascin polypeptides in immunoprecipitation, or just the Mr-250,000 polypeptide in immunoblots. It can also interact with the fibrinogen-like knob domain of the tenascin protein, especially in paraffin sections of human tissue. It can be used in immunofluorescence, immunoblotting and immunohistochemistry on formalin-fixed or paraffin-embedded sections. Tenascin polypeptides isolated from the spent culture supernatant of human fibroblasts by affinity chromatography are used as immunogens for antibody production.

Plasma fibronectin

This MAb interacts with both cellular and plasma forms of fibronectin, and has an affinity for cellular binding regions. It can cross-react with humans and guinea pigs. This antibody can be used in immunohistochemical staining, western blotting, and other immunoassay methods for cellular and tumour biology. Purified fibronectin from A8387 fibrosarcoma cells is used as an immunogen for antibody production.

Pepsinogen II

This human gastric biomarker MAb is specific to human pepsinogen II, with no cross-reactivity to human pepsinogen I. It is suitable for immunohistochemical staining, western blotting and enzyme immunoassays. Pepsinogen II – purified from human tissue – is used as an immunogen for antibody production.

Pepsinogen I

This human gastric biomarker MAb is specific to human pepsinogen I, with no cross-reactivity to human pepsinogen II. It is suitable for immunohistochemical staining, western blotting and enzyme immunoassays. Pepsinogen I – purified from human tissue – is used as an immunogen for antibody production.

PECAM-1

This MAb to human endothelial cell surface markers interacts with Mr-135,000 PECAM-1 polypeptides from leukocytes and endothelial cells. It can also detect all breast endothelial cells, and identifies erythroid differentiation in human leukemic cells. It can be used in immunohistochemical staining, western blotting and other immunoassays for cellular and tumour biology. Ulex Europaeus-agglutinin-I reactive glycoproteins – taken from cultured human umbilical vein endothelial cells – were isolated by lecithin-affinity chromatography and used as immunogens for antibody production.

Neurofilaments 70, 200

This human cytoskeletal polypeptide MAb interacts with Mr-68,000 and Mr-200,000 neurofilament proteins in human tissues. It can be used in immunohistochemical staining and western blotting. Purified neurofilament triplet proteins from the human spinal cord are used as immunogens for antibody production.

Neurofilaments 150, 200

This cytoskeletal polypeptide MAb interacts with all three neurofilament protein molecules – Mr 68,000, Mr-150,000 and Mr-200,000 – in mice, as well as Mr-150,000 and Mr-200,000 in human tissues. It can be used in immunohistochemical staining and western blotting. Purified neurofilament triplet proteins from human spinal cords were used as immunogens to produce this MAb.

Laminin – gamma 1-chain

This MAb interacts with the Mr-200,000 γ1-chain of human-reduced and alkylated laminin; a protein synthesised by cultured cells. The specificity of this antibody was confirmed by immunoprecipitation and ELISA, using a recombinant γ1-chain. It only reacts with human cells and tissues and, although it does not work in western blotting, it can be used in immunohistochemical staining and immunoprecipitation. Pepsin-digested laminins from human placenta are used as immunogens for antibody production.

Laminin – beta 1-chain

This MAb interacts with the NH2-terminus of the β1-chain subunit of laminin with a molecular mass (Mr) of 220,000. It can be used in human tissues for immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology. This clone reacts strongly both under reducing and non reducing conditions. It can also be successfully used to purify laminin-8 and laminin-10 using affinity chromatography, unlike many other antibodies to the laminin β1-chain. Partially pepsin-digested laminins from human placenta are used as immunogens for antibody production.

Genistein MAb

This monoclonal antibody recognises and interacts with human genistein. There is some cross-reactivity with daidzein (3.3 %), dihydrodaidzein (2.5 %), dihydrogenistein (0.4 %), and glycitein (0.3 %), but none with equol or O-DMA. It can be used for enzyme or fluorescent immunoassays, and is produced using BSA-conjugated human genistein as an immunogen.

Gastrin-17 MAb

This monoclonal antibody is specific to the human gastric biomarker protein hormone gastrin-17, though it has no cross-reactivity to gastrin-34 or cholecystokinin. It can be used in immunohistochemical staining and enzyme immunoassays. Synthetic gastrin-17 coupled to thyroglobulin is used as an immunogen for antibody production.

Gamma-aminobutyric acid (GABA) MAb

This monoclonal antibody is highly specific to the human neurotransmitter, GABA, with a weak interaction against the β-alanine BSA-conjugate antigen, but only at the highest concentrations. There is no interaction with any other amino acid-BSA conjugates. It can be used in fluorescence microscopy and other immunohistochemical methods, as well as on frozen sections, provided they have been perfusion fixed beforehand. Glutaraldehyde must be included in the fixative of paraffin sections, if they are used. GABA coupled to BSA with glutaraldehyde is used as an immunogen for antibody production.

Erythroid beta-spectrin MAb

This monoclonal antibody is specific to the 220 kD human erythroid β-spectrin protein, present in skeletal muscle cell membranes. It can be used to identify erythroid leukaemia, to monitor the integrity of muscle cell plasma membranes, and in various immunochemical reactions, immunoprecipitation, western blotting, as well as immunocytochemistry methodologies. Ghost proteins from human red blood cells are used as immunogens for production of this antibody.

Erythroid alpha-spectrin MAb

This monoclonal antibody is specific to the 240 kD human erythroid α-spectrin protein. It can be used to detect erythroid spectrin or to identify erythroid leukaemia, and also in various immunochemical reactions, immunoprecipitation, western blotting, as well as immunocytochemistry methodologies. Ghost proteins from human red blood cells are used as immunogens for production of this antibody.

Cytokeratin 8, 18 and 19 MAb

This monoclonal antibody interacts with the cytokeratin 8, 18 and 19 genes, found in many simple epithelial cells, and it can be used in Immunohistochemical staining and western blotting. Purified cytokeratin – from cultured pig kidney epithelial cells – is used as an immunogen for production of this antibody.

Cytokeratin 7, 17 and 19 MAb

This monoclonal antibody interacts with the cytokeratin 7, 17 and 19 genes, found in many simple and squamous epithelial cells, and it can be used in immunohistochemical staining and western blotting. Purified cytokeratin – from cultured pig kidney epithelial cells – is used as an immunogen for production of this antibody.

Cytokeratin 18 MAb

This monoclonal antibody identifies the cytokeratin 18 gene (CK18), coding for a type I intermediate filament protein found mainly in simple epithelial cells. It interacts with a variety of different species, especially mouse CK18, and can be applied in developmental biological and gene knockout studies, as well as for immunohistochemical staining, western blotting and immunoprecipitation. Cytokeratin – isolated from cultured pig kidney epithelial cells – is used as an immunogen for production of this antibody.

Calcitonin gene related peptide (CGRP) MAb

This MAb is highly specific to CGRPs, remaining inactive with other neuropeptides. It functions within human and guinea pig tissue, but not rat. It can be used in ELISA, fluorescence microscopy, immunoblotting with BSA-conjugated samples, and other immunohistochemical methods, as well as on frozen sections providing they have undergone perfusion fixation. Paraformaldehyde must be included in the fixative with paraffin sections, if used. A terminal fragment of the CGRP, coupled to BSA with glutaraldehyde, is used as an immunogen for antibody production.

Cellular fibronectin MAb

This MAb is specific to the extradomain A sequence of human fibronectin. It cross-reacts with humans, rats, chickens, rabbits and guinea pigs, and can be used in immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology. Purified fibronectin from A8387 fibrosarcoma cells is used as an immunogen for antibody production.

Beta 3-integrin MAb

Monoclonal antibody to the β3 human integrin, including the GPIIIa complex on platelets.

Beta 1-integrin – IgG1, clone DF5 MAb

Monoclonal antibody to the extracellular β1 subunit epitope of the human integrin protein family.

Beta 1-integrin – IgG1, clone DF7 MAb

Monoclonal antibody to human extracellular β1 subunit epitope of the integrin protein family.

Alpha-fodrin MAb

Monoclonal antibody to the 240 kD human cytoskeletal polypeptide α-fodrin.

Alpha-actinin MAb

Monoclonal antibody to the 100 kD human cytoskeletal polypeptide α-actinin.

Alpha II beta-Integrin MAb

Monoclonal antibody to human αIIβ-subunit of β-integrins.

GastroPanel® Reader

Point of care test

The GastroPanel Reader NT is the immunofluorescent lateral flow test analyser used in combination with the GastroPanel quick test NT, enabling the rapid and precise measurement and interpretation of the test results.

BIOHIT Faecal Calprotectin Extraction Tubes

BIOHIT Faecal Calprotectin Extraction Tubes are intended for the extraction of human stool samples for measurement of calprotectin using the BIOHIT Calprotectin ELISA.

BIOHIT FAEX® Sample System

The BIOHIT FAEX™ Sample System is a multi-functional stool sample collection, handing and storage device, for assays that require precise and simple stool shipping and extraction.

GastroPanel® Quick Test

Point of care test

GastroPanel® Quick Test helps clinicians triage patients with stomach symptoms before gastroscopy, identifying who needs further investigation and who doesn't. A finger-prick blood test with results in 15 minutes, designed for use at the point of care.

Automated ELISA analyser accessories

BIOHIT supplies various accessories for its ELISA automate systems, offering flexible customisation to suit a range of assays.

Dynex DSX 4-plate Automated ELISA processor

BIOHIT’s DSX is a fully automated, four-plate processing system that can perform multiple assays per plate. The DSX’s modular design provides flexible configuration and was developed with ease of use in mind. The system incorporates many features that ensure the quality and security of results, and it can handle a wide variety of assays, offering reliable sample-in/result-out processing for true walkaway automation.

Dynex DS2® 2-plate automated ELISA processor with barcode reader

BIOHIT’s DS2 automated ELISA processor offers full walkaway capability, allowing users to quickly and easily process two 96-well microplates and up to 12 different assays simultaneously. It features a user-friendly control system, chain of custody management and on-board instrument diagnostics for straightforward, reliable operation and traceability.

GastroPanel® Helicobacter pylori IgG ELISA

GastroPanel Helicobacter pylori test is an in vitro microplate-based quantitative enzyme-linked immunosorbent assay (ELISA) for the determination of IgG antibodies against H. pylori from EDTA plasma samples. This kit is part of the GastroPanel family of products.

GastroPanel® Unified (Pepsinogen I, Pepsinogen II, H. pylori IgG and Gastrin-17 ELISAs)

GastroPanel® Unified ELISA is a non-invasive triage test for individuals with stomach symptoms prior to gastroscopy. It comprises four quantitative biomarker assays that screen for and diagnose clinically relevant atrophic gastritis and Helicobacter pylori infection to help evaluate gastric cancer risk. The four biomarker assays are pepsinogen I, pepsinogen II, gastrin-17, and Helicobacter pylori antibodies, collectively designed to give information on both the structure and function of the stomach mucosa from a serum or plasma sample.

GastroPanel® Gastrin-17 ELISA

GastroPanel Gastrin-17 kit is a microplate-based quantitative ELISA for the determination of human amidated gastrin-17 from plasma or serum samples. This test identifies Helicobacter pylori-infected patients who have advanced atrophic gastritis in the gastric antrum. This kit is part of the GastroPanel family of products.

BIOHIT ColonView® quick test (FIT)

BIOHIT ColonView quick test is a new generation FIT test that detects both haemoglobin (Hb) and haemoglobin/haptoglobin (Hb/Hp) complexes in stool samples.

BIOHIT Helicobacter pylori Control

The BIOHIT H. pylori CONTROL+ is a quality control for rapid urease tests. Used in conjunction with biopsy tests for H. pylori in endoscopy settings H. pylori CONTROL+ provides rapid quality assurance in minutes, delivering confidence in testing and confidence in results.

Helicobacter pylori quick test

The BIOHIT Helicobacter pylori quick test is a rapid, one-step urease test to detect H. pylori infection in biopsy samples during gastroscopy. It can also be used to evaluate the success of eradication therapy. This easy-to-use, gel-based test employs a simple colour indicator that allows easy visual confirmation of positive results in as little as two minutes – and negative results after 30 minutes – without specialist training.

BIOHIT Helicobacter pylori UFT300

The BIOHIT UFT300 Helicobacter pylori quick test is an ultra-fast urease test that has revolutionised the way H. pylori infection is diagnosed and reported in gastroscopy. With extremely high accuracy and every result available within five minutes, this ready-to-use test is the choice for endoscopists.

BIOHIT Total 25OH vitamin D ELISA

The BIOHIT Total 25OH Vitamin D ELISA is a quantitative immunoenzymatic assay for the determination of 25-hydroxyvitamins D2 and D3 in serum and plasma, to aid the diagnosis of vitamin D deficiency, insufficiency or intoxication.

BIOHIT Calprotectin ELISA

The BIOHIT Calprotectin ELISA is a quantitative sandwich ELISA method for the detection of calprotectin in stool samples. It can be used to help identify organic diseases of the small or large intestine, and to monitor disease activity and response to treatment in patients with ulcerative colitis or Crohn’s disease.

BIOHIT Active B12 (holotranscobalamin) ELISA

The BIOHIT Active B12 (holotranscobalamin) ELISA directly measures holotranscobalamin (holoTC) – the biochemically active form of vitamin B12 – from human serum. This test is well-suited for the screening of patients with a suspected vitamin B12 deficiency, and overcomes the problems associated with traditional testing methods that measure total, rather than just active, vitamin B12 concentration.

BIOHIT Celiac Quick Test

The BIOHIT Celiac Quick Test is a rapid immunochromatographic test for the qualitative detection of antibodies (IgA, IgG, IgM) against human tissue transglutaminase (tTG2A) in capillary whole blood samples.

Lactose Intolerance quick test

The BIOHIT Lactose Intolerance Quick Test is a rapid enzymatic test for the determination of lactase in duodenal biopsies. Uned in endoscopy in conjunction with OGD (upper endoscopy) the Lactose Intolerance quick test supports the diagnosis of hypolactasia of the small intestine from a single biopsy specimen.

GastroPanel® Pepsinogen II ELISA

GastroPanel Pepsinogen II kit is a microplate-based quantitative ELISA for the determination of human pepsinogen II from plasma or serum samples. This test is intended as an additional tool in the diagnosis of atrophic corpus gastritis, which is also a risk state for gastric cancer. This kit is part of the GastroPanel family of products.

GastroPanel® Pepsinogen I ELISA

GastroPanel Pepsinogen I kit is a microplate-based quantitative ELISA for the determination of human pepsinogen I from plasma or serum samples. This test is intended to identify patients who have advanced atrophic gastritis in the gastric corpus, and are therefore at increased risk of developing gastric cancer. This kit is part of the GastroPanel family of products.