Monoclonal Antibodies

This MAb is specific to human tissue extracellular matrix components such as vitronectin, with no cross-reaction with other matrix molecules. It can be used in immunohistochemical staining, immunoblotting and other immunoassays for cellular and tumour biology research. Purified vitronectin from human plasma is used as an immunogen for antibody production.

This human cytoskeletal polypeptide MAb can be used in immunoblotting, immunohistochemical staining and other immunoassays for cellular and tumour biology research. In immunoblotting, it identifies a 130 kD polypeptide. Vinculin, purified from human smooth muscle tissue, is used as an immunogen for antibody production.

This human cytoskeletal polypeptide MAb recognises vimentin with an Mr of 57,000 in mesenchymal cells, and can be used in immunohistochemical staining and western blotting. Purified vimentin, obtained via electrophoresis from cultured human fibroblasts, is used as an immunogen for antibody production.

This human extracellular MAb interacts with the fourth and fifth fibronectin-like repeats of human tenascin-C polypeptides with an Mr of 250,000 and 180,000. It can cross-react with humans, guinea pigs and rabbits. It can also be used in immunohistochemical staining, western blotting and other immunoassays for cellular and tumour biology research, as well as for quantitation of Tenascin-C in body fluids. Purified tenascin-C from human fetal fibroblasts is used as an immunogen for antibody production.

This MAb identifies the Mr-250,000 and Mr-180,000 human tenascin polypeptides in immunoprecipitation, or just the Mr-250,000 polypeptide in immunoblots. It can also interact with the fibrinogen-like knob domain of the tenascin protein, especially in paraffin sections of human tissue. It can be used in immunofluorescence, immunoblotting and immunohistochemistry on formalin-fixed or paraffin-embedded sections. Tenascin polypeptides isolated from the spent culture supernatant of human fibroblasts by affinity chromatography are used as immunogens for antibody production.

This MAb interacts with both cellular and plasma forms of fibronectin, and has an affinity for cellular binding regions. It can cross-react with humans and guinea pigs. This antibody can be used in immunohistochemical staining, western blotting, and other immunoassay methods for cellular and tumour biology. Purified fibronectin from A8387 fibrosarcoma cells is used as an immunogen for antibody production.

This human gastric biomarker MAb is specific to human pepsinogen II, with no cross-reactivity to human pepsinogen I. It is suitable for immunohistochemical staining, western blotting and enzyme immunoassays. Pepsinogen II – purified from human tissue – is used as an immunogen for antibody production.

This human gastric biomarker MAb is specific to human pepsinogen I, with no cross-reactivity to human pepsinogen II. It is suitable for immunohistochemical staining, western blotting and enzyme immunoassays. Pepsinogen I – purified from human tissue – is used as an immunogen for antibody production.

This MAb to human endothelial cell surface markers interacts with Mr-135,000 PECAM-1 polypeptides from leukocytes and endothelial cells. It can also detect all breast endothelial cells, and identifies erythroid differentiation in human leukemic cells. It can be used in immunohistochemical staining, western blotting and other immunoassays for cellular and tumour biology. Ulex Europaeus-agglutinin-I reactive glycoproteins – taken from cultured human umbilical vein endothelial cells – were isolated by lecithin-affinity chromatography and used as immunogens for antibody production.

This human cytoskeletal polypeptide MAb interacts with Mr-68,000 and Mr-200,000 neurofilament proteins in human tissues. It can be used in immunohistochemical staining and western blotting. Purified neurofilament triplet proteins from the human spinal cord are used as immunogens for antibody production.

This cytoskeletal polypeptide MAb interacts with all three neurofilament protein molecules – Mr 68,000, Mr-150,000 and Mr-200,000 – in mice, as well as Mr-150,000 and Mr-200,000 in human tissues. It can be used in immunohistochemical staining and western blotting. Purified neurofilament triplet proteins from human spinal cords were used as immunogens to produce this MAb.

This MAb interacts with the Mr-200,000 γ1-chain of human-reduced and alkylated laminin; a protein synthesised by cultured cells. The specificity of this antibody was confirmed by immunoprecipitation and ELISA, using a recombinant γ1-chain. It only reacts with human cells and tissues and, although it does not work in western blotting, it can be used in immunohistochemical staining and immunoprecipitation. Pepsin-digested laminins from human placenta are used as immunogens for antibody production.

This MAb interacts with the NH2-terminus of the β1-chain subunit of laminin with a molecular mass (Mr) of 220,000. It can be used in human tissues for immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology. This clone reacts strongly both under reducing and non reducing conditions. It can also be successfully used to purify laminin-8 and laminin-10 using affinity chromatography, unlike many other antibodies to the laminin β1-chain. Partially pepsin-digested laminins from human placenta are used as immunogens for antibody production.

This MAb recognises and interacts with human genistein. There is some cross-reactivity with daidzein (3.3 %), dihydrodaidzein (2.5 %), dihydrogenistein (0.4 %), and glycitein (0.3 %), but none with equol or O-DMA. It can be used for enzyme or fluorescent immunoassays, and is produced using BSA-conjugated human genistein as an immunogen.

This MAb is specific to the human gastric biomarker protein hormone gastrin-17, though it has no cross-reactivity to gastrin-34 or cholecystokinin. It can be used in immunohistochemical staining and enzyme immunoassays. Synthetic gastrin-17 coupled to thyroglobulin is used as an immunogen for antibody production.

This MAb is highly specific to the human neurotransmitter, GABA, with a weak interaction against the β-alanine BSA-conjugate antigen, but only at the highest concentrations. There is no interaction with any other amino acid-BSA conjugates. It can be used in fluorescence microscopy and other immunohistochemical methods, as well as on frozen sections, provided they have been perfusion fixed beforehand. Glutaraldehyde must be included in the fixative of paraffin sections, if they are used. GABA coupled to BSA with glutaraldehyde is used as an immunogen for antibody production.

This MAb is specific to the 220 kD human erythroid -spectrin protein, present in skeletal muscle cell membranes. It can be used to identify erythroid leukaemia, to monitor the integrity of muscle cell plasma membranes, and in various immunochemical reactions, immunoprecipitation, western blotting, as well as immunocytochemistry methodologies. Ghost proteins from human red blood cells are used as immunogens for production of this antibody.

This MAb is specific to the 240 kD human erythroid α-spectrin protein. It can be used to detect erythroid spectrin or to identify erythroid leukaemia, and also in various immunochemical reactions, immunoprecipitation, western blotting, as well as immunocytochemistry methodologies. Ghost proteins from human red blood cells are used as immunogens for production of this antibody.

This MAb interacts with the cytokeratin 8, 18 and 19 genes, found in many simple epithelial cells, and it can be used in Immunohistochemical staining and western blotting. Purified cytokeratin – from cultured pig kidney epithelial cells – is used as an immunogen for production of this antibody.

This MAb interacts with the cytokeratin 7, 17 and 19 genes, found in many simple and squamous epithelial cells, and it can be used in immunohistochemical staining and western blotting. Purified cytokeratin – from cultured pig kidney epithelial cells – is used as an immunogen for production of this antibody.

This MAb identifies the cytokeratin 18 gene (CK18), coding for a type I intermediate filament protein found mainly in simple epithelial cells. It interacts with a variety of different species, especially mouse CK18, and can be applied in developmental biological and gene knockout studies, as well as for immunohistochemical staining, western blotting and immunoprecipitation. Cytokeratin – isolated from cultured pig kidney epithelial cells – is used as an immunogen for production of this antibody.

This MAb is highly specific to CGRPs, remaining inactive with other neuropeptides. It functions within human and guinea pig tissue, but not rat. It can be used in ELISA, fluorescence microscopy, immunoblotting with BSA-conjugated samples, and other immunohistochemical methods, as well as on frozen sections providing they have undergone perfusion fixation. Paraformaldehyde must be included in the fixative with paraffin sections, if used. A terminal fragment of the CGRP, coupled to BSA with glutaraldehyde, is used as an immunogen for antibody production.

This MAb is specific to the extradomain A sequence of human fibronectin. It cross-reacts with humans, rats, chickens, rabbits and guinea pigs, and can be used in immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology. Purified fibronectin from A8387 fibrosarcoma cells is used as an immunogen for antibody production.

This MAb interacts with the β3 human integrin, including the GPIIIa complex on platelets. It can be used for immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology research. A partially purified platelet glycoprotein IIb/IIIa complex is used as an immunogen for production of this antibody.

This MAb interacts with the extracellular β1 subunit epitope of the human integrin protein family. It can be used for immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology research, as well as immunofluorescence staining of paraffin sections. Wheat germ agglutinin-binding glycoproteins from human fibroblasts are used as immunogens for antibody production.

This MAb to human integrins interacts with the extracellular β1 subunit epitope of the integrin protein family. It can be used for immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology research, as well as immunofluorescence staining of paraffin sections. Wheat germ agglutinin-binding glycoproteins from human fibroblasts are used as immunogens for antibody production.

This is a MAb specific to the 240 kD human cytoskeletal polypeptide α-fodrin of all non-erythroid mammalian cells, as well as α-spectrin in chickens. It cross-reacts with humans, rats, chickens, rabbits and guinea pigs, and can be used for immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology research. Red blood cell membranes from chickens are purified by hypotonic lysis and mechanical enucleation, and used as immunogens for antibody production.

This is a MAb specific to the 100 kD human cytoskeletal polypeptide α-actinin, interacting with human and chicken cells. It can be used for immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology research. Purified alpha-actinin from chicken gizzard is used as an immunogen for production of antibodies.

The MAb to human integrins can be used for immunohistochemical staining, immunoblotting and other immunoassays for cellular or tumour biology research. It interacts with the IIb-subunit of β-integrins, specifically on platelets, megakaryocytes and leukemic cells that undergo megakaryocytic differentiation. A partially purified platelet glycoprotein IIb/IIIa complex is used as an immunogen for antibody production.